A Comprehensive scRNASeq Analysis Pipeline Using Scanpy and Associated Tools

This white paper presents a single-cell transcriptomic pipeline map of the bladder cancer microenvironment, integrating multiple GEO datasets. The study reveals major shifts in immune and stromal cell populations, activation of oncogenic and immunosuppressive pathways, and identifies TP53, EGFR, MYC, and STAT3 as key therapeutic targets.